Author/Editor		Stražišar, Mojca; Rott, Tomaž; Glavač, Damjan
Title		K-RAS and P53 mutations in association with COX-2 and hTERT expression and clinico-pathological status of NSCLC patients
Type		članek
Source		Dis Markers
Vol. and No.		Letnik 25, št. 2
Publication year		2008
Volume		str. 97-106
Language		eng
Abstract		We evaluated the occurrence of mutations in P53, K-RAS, COX-2, expression of COX-2 and hTERT and relations among clinicopathological signs. P53 mutations were identified in 34.4% of tumours, the majority of them occurring in SCC (squamous cell carcinoma, 55.6%). K-RAS was mutated in 12.2% of NSCLC tumours, the majority of the mutations being found in ADC (adenocarcinoma, 27.0%). Mutational screening detected three different COX-2 mutations and five different P53 mutations, published for the first time. With RT-PCR we observed that the expression of the tested genes, hTERT and COX-2, was highly significant for ADC (p < 0.01) and SCC (p < 0.05). Statistical analysis of the combined results revealed significant correlation between expression of COX-2 and hTERT (p < 0.001), hTERT expression and staging (p < 0.05) and survival (p < 0.01). A positive correlation between COX-2 expression and K-RAS mutation (p <0.05) was also observed. This study provides insight into associations between the analysed biomarkers and the clinical-pathological data of the patients.
Descriptors		LUNG NEOPLASMS MUTATION GENES, P53 GENES, RAS GENE EXPRESSION KI-67 ANTIGEN CARCINOMA, NON-SMALL-CELL LUNG ADENOCARCINOMA CARCINOMA, SQUAMOUS CELL CARCINOMA, LARGE CELL POLYMERASE CHAIN REACTION PROSTAGLANDIN-ENDOPEROXIDE SYNTHASE