Author/Editor		Smerdu, Vika
Title		Postnatalni razvoj skeletne mišice prašiča: vpliv na histokemične in morfometrične lastnosti mišičnih vlaken
Type		monografija
Place		Ljubljana
Publisher		Medicinska fakulteta
Publication year		1993
Volume		str. 67
Language		slo
Abstract		The samples of longissimus dorsi (LD), rectus femoris (RF) and vastus intermedius (VI) muscles of newborn, three week and six month old(adult) pigs have been histochemically stained to show muscle fibre types. Numerical density (NN), areal density (AA), average diameter ( D ) and spatial distribution of type I, IIA, IIB and IIC fibres have been determined. Metabolical properties of muscle fibre types have been established according to the activities of oxidative (NADH-TR) and glycolytical (a-GPDH) enzymes. Subsequently an immunohistochemical analysis of the same three muscles of adult animals was performed by using monoclonal antibodies to myosin heavy chains (MHC) I, IIA and IIB. Oxidative metabolism prevails in all muscle fibre types of newborn piglets. LD and RF are both white glycolytical muscles, where type IIA fibres predominate at newborns, but later they transform to type IIB fibres. They are mostly larger, more glycolytical and prevail at adults. The percentage of type IIC fibres, which are specific for developing muscles, is small. This type of fibres is present only up to the age of three weeks. In next five months of animal's life there are no considerable changes in fiber types' percentages, so it is persumed that LD and RF muscles are relatively mature at that age. The VI is red oxidative muscle which undergoes intensive changes all time after birth: the percentage of oxidative type I fibres increases constantly and prevails at adults. Type II fibres are more oxidative in VI than in the other two muscles. twice as large as that of type II fibres. The presence of type aC fibres in VI muscle of six month old pigs indicates that the fibre type transformation has not yet been ćompleted at that age. In all three muscles there are no significant differences between NN and AA of each fibre type. Except in LD muscle of newborn pigs, where AA of type I fibres is three times higher than their NN.(trunc.)
Descriptors		MUSCLES HISTOCYTOCHEMISTRY ADENOSINETRIPHOSPHATASE NADH TETRAZOLIUM REDUCTASE GLYCEROLPHOSPHATE DEHYDROGENASE ANIMALS, DOMESTIC AGE FACTORS SWINE IMMUNOHISTOCHEMISTRY MYOSIN BIOMETRY