Author/Editor		Uršič, Viktor; Tomič, Viktorija; Košnik, Mitja
Title		Effect of different incubation atmospheres on the production of biofilm in methicillin-resistant staphylococcus aureus (MRSA) grown in nutrient-limited medium
Type		članek
Source		Curr Microbiol
Vol. and No.		Letnik 57, št. 4
Publication year		2008
Volume		str. 386-90
Language		eng
Abstract		Today, methicillin-resistant Staphylococcus aureus (MRSA) is a feared cause of nosocomial infections worldwide. These organisms can gain increased resistance to antimicrobial agents through biofilm formation, which appears to be a bacterial survival strategy. MRSA isolates obtained from patients were cultured in nutrient-limited medium supplemented with 0.2% glucose in aerobic, anaerobic, and CO(2) incubation atmospheres. Biofilm formation was quantified by the microtiter plate test. MRSA strains showed significantly lower biofilm production when grown in an aerobic atmosphere compared to that exhibited in CO(2)-rich environments. Gaseous conditions and growth in a nutritionally limited medium can profoundly influence the amount of biofilm formation in MRSA. This should be considered in any in vitro study of in vivo behavior.
Descriptors		CROSS INFECTION STAPHYLOCOCCUS AUREUS METHICILLIN RESISTANCE CULTURE MEDIA, CONDITIONED BIOFILMS