Author/Editor     Lokar, Maruša; Urbanija, Jasna; Frank, Mojca; Hagerstrand, Henry; Rozman, Blaž; Bobrowska-Hagerstrand, Malgorzata; Iglič, Aleš; Kralj-Iglič, Veronika
Title     Agglutination of like-charged red blood cells induced by binding of beta(2)-glycoprotein I to outer cell surface
Type     članek
Source     Bioelectrochemistry
Vol. and No.     Letnik 73, št. 2
Publication year     2008
Volume     str. 110-6
Language     eng
Abstract     Plasma protein-mediated attractive interaction between membranes of red blood cells (RBCs) and phospholipid vesicles was studied. It is shown that beta(2)-glycoprotein I (beta(2)-GPI) may induce RBC discocyte-echinocyte-spherocyte shape transformation and subsequent agglutination of RBCs. Based on the observed beta(2)-GPI-induced RBC cell shape transformation it is proposed that the hydrophobic portion of beta(2)-GPI molecule protrudes into the outer lipid layer of the RBC membrane and increases the area of this layer. It is also suggested that the observed agglutination of RBCs is at least partially driven by an attractive force which is of electrostatic origin and depends on the speci fi c molecular shape and internal charge distribution of membrane-bound beta(2)-GPI molecules. The suggested beta(2)-GPI-induced attractive electrostatic interaction between like-charged RBC membrane surfaces is qualitatively explained by using a simple mathematical model within the functional density theory of the electric double layer, where the electrostatic attraction between the positively charged part of the first domains of bound beta(2)-GPI molecules and negatively charged glycocalyx of the adjacent RBC membrane is taken into account.
Descriptors     ERYTHROCYTE AGGREGATION
GLYCOPROTEINS
BINDING SITES
CELL MEMBRANE
LIPOSOMES
PHOSPHOLIPIDS
MODELS, THEORETICAL