Author/Editor     Injac, Rade; Perše, Martina; Obermajer, Nataša; Djordjević -Milić, Vukosava; Prijatelj, Matevž; Djordjević, Aleksandar; Cerar, Anton; Štrukelj, Borut
Title     Potential hepatoprotective effects of fullerenol C60(OH)24 in doxorubicin-induced hepatotoxicity in rats with mammary carcinomas
Type     članek
Source     Biomaterials
Vol. and No.     Letnik 29, št. 23-24
Publication year     2008
Volume     str. 3451-60
Language     eng
Abstract     The aim of this study was to investigate the potential protective role of fullerenol C60(OH)24 on doxorubicin-induced liver toxicity using in vivo (female Sprague-Dawley rats) and in vitro (human hepatocellular carcinoma - HepG2č colorectal adenocarcinoma cell lines - Caco-2) approaches. The first (healthy control) and second (control with chemically induced mammary carcinomas) group received saline only. The third, fourth and fifth group (allwith breast cancer) were injected (i.p.) with a single dose of doxorubicin(8 mg/kg), doxorubicin/fullerenol (100 mg/kg of fullerenol 30 min before administration of 8 mg/kg doxorubicin) and fullerenol (100 mg/kg), respectively. Two days after treatment, the rats were sacrificed. Results showed that treatment with doxorubicin alone caused significant changes in theserum levels of alanine aminotransferase (ALT), aspartate aminotransferase (AST), lactate dehydrogenase (LDH) and -hydroxybutyrate dehydrogenase ( -HBDH), as well as in the levels of malondialdehyde (MDA), glutathione (GSH), glutathione peroxidase (GSH-Px), total antioxidant status (TAS), glutathione reductase (GR), catalase (CAT) and superoxide dismutase (SOD) in the liver tissue. These effects were significantly reduced for all investigated parameters by pre-treatment with fullerenol but not for the MDA and GSH level.The HepG2 and Caco-2 cell lines were continuously treated with g/mL and 44 micro g/mL. With the aim of evaluating the modulating activity of fullerenol on doxorubicin-induced hepatotoxicity, the cell lines were simultaneously m) and fullerenol (10 microg/mL/ 44 microg/mL) in different m doxorubicin alongwith the fullerenol, we can see a significant improvement of the cell capability during the entire time-line. We can conclude that fullerenol has cytotoxic effects on HepG2 by itself, but when the oxidative stress is too high the cytotoxic effects of fullerenol are overcome by its protective role as a strong antioxidant compound.