Author/Editor		Gostinčar, C; Turk, M; Trbuha, T; Vaupotič, T; Plemenitaš, A; Gunde-Cimerman, N
Title		Expression of fatty-acid-modifying enzymes in the halotolerant black yeast Aureobasidium pullulans (de Bary) G. Arnaud under salt stress
Type		članek
Source		Stud Mycol
Vol. and No.		Letnik 61
Publication year		2008
Volume		str. 51-9
Language		eng
Abstract		Multiple tolerance to stressful environmental conditions of the black, yeast-like fungus Aureobasidium pullulans is achieved through different adaptations, among which there is the restructuring of the lipid composition of their membranes. Here, we describe three novel genes encoding fatty-acid-modifying enzymes in A. pullulans, along with the levels of their mRNAs under different salinity conditions. High levels of 9-desaturase and 12-desaturase mRNAs were seen at high salinities, which were consistent with an increased desaturation of the fatty acids in the cell membranes. Elevated levels of elongase mRNA were also detected. Surprisingly, increases in the levels of these mRNAs were also seen following hypo-osmotic shock, while hyperosmotic shock had exactly the opposite effect, demonstrating that data that are obtained from up-shift and down-shift salinity studies should be interpreted with caution.
Descriptors		YEASTS SODIUM CHLORIDE CULTURE MEDIA MEMBRANE LIPIDS RNA, MESSENGER OLIGONUCLEOTIDE PROBES POLYMERASE CHAIN REACTION PHYLOGENY FATTY ACID DESATURASES