Author/Editor		Fekete, Veronika; Čierna, Maria; Polakova, Silvia; Piškur, Jure; Sulo, Pavol
Title		Transition of the ability to generate petites in the Saccharomyces/Kluyveromyces complex
Type		članek
Source		FEMS Yeast Res
Vol. and No.		Letnik 7, št. 8
Publication year		2007
Volume		str. 1237-47
Language		eng
Abstract		Petite-positivity - the ability to tolerate the loss of mtDNA - was examined after the treatment with ethidium bromide (EB) in over hundred isolates from the Saccharomyces/Kluyveromyces complex. The identity of petite mutants was confirmed by the loss of specific mtDNA DAPI staining patterns. Besides unequivocal petite-positive and petite-negative phenotypes, a few species exhibited temperature sensitive petite positive phenotype and petiteness of a few other species could be observed only at the elevated EB concentrations. Several yeast species displayed a mixed 'moot' phenotype, where a major part of the population did not tolerate the loss of mtDNA but several cells did. The genera from postwhole-genome duplication lineages (Saccharomyces, Kazachstania, Naumovia, Nakaseomyces) were invariably petite-positive. However, petite-positive traits could also be observed among the prewhole-genome duplication species.
Descriptors		DNA, FUNGAL DNA, MITOCHONDRIAL ETHIDIUM GENOME, FUNGAL KLUYVEROMYCES MITOCHONDRIA MUTAGENESIS MUTAGENS PHENOTYPE PHYLOGENY SACCHAROMYCES