Author/Editor     Zameitat, Elke; Knecht, Wolfgang; Piškur, Jure; Loffler, Monika
Title     Two different dihydroorotate dehydrogenases from yeast Saccharomyces kluyveri
Type     članek
Source     Febs Lett
Vol. and No.     Letnik 568, št. 1-3
Publication year     2004
Volume     str. 129-34
Language     eng
Abstract     Genes for two structurally and functionally different dihydroorotate dehydrogenases (DHODHs, EC 1.3.99.11), catalyzing the fourth step of pyrimidine biosynthesis, have been previously found in yeast Saccharomyces kluyveri. One is closely related to the Schizosaccharomyces pombe mitochondrial family 2 enzymes, which use quinones as direct and oxygen as the final electron acceptor. The other one resembles the Saccharomyces cerevisiae cytosolic family 1A fumarate-utilizing DHODH. The DHODHs from S. kluyveri, Sch. pombe and S. cerevisiae, were expressed in Escherichia coli and compared for their biochemical properties and interaction with inhibitors. Benzoates as pyrimidine ring analogs were shown to be selective inhibitors of cytosolic DHODs. This unique property of Saccharomyces DHODHs could appoint DHODH as a species-specific target for novel anti-fungal therapeutics.
Descriptors     BASE SEQUENCE
DNA PRIMERS
ELECTROPHORESIS, POLYACRYLAMIDE GEL
ISOENZYMES
KINETICS
RECOMBINANT PROTEINS
SACCHAROMYCES