| Author/Editor | Dobritzsch, Doreen; Andersen, Birgit; Piškur, Jure | |
| Title | Crystallization and X-ray diffraction analysis of dihydropyrimidinase from Saccharomyces kluyveri | |
| Type | članek | |
| Source | Acta Crystallogr Sect F Struct Biol Cryst Commun | |
| Vol. and No. | Letnik 61, št. Pt 4 | |
| Publication year | 2005 | |
| Volume | str. 359-62 | |
| Language | eng | |
| Abstract | Dihydropyrimidinase (EC 3.5.2.2) catalyzes the second step in the reductive pathway of pyrimidine degradation, the hydrolysis of 5,6-dihydrouracil and 5,6-dihydrothymine to the corresponding N-carbamylated beta-amino acids. Crystals of the recombinant enzyme from the yeast Saccharomyces kluyveri diffracting to 2.6 A at a synchrotron-radiation source have been obtained by the hanging-drop vapour-diffusion method. They belong to space group P2(1) (unit-cell parameters a = 91.0, b = 73.0, c = 161.4 A, beta = 91.4 degrees), with one homotetramer per asymmetric unit. | |
| Descriptors | AMIDOHYDROLASES CRYSTALLIZATION SACCHAROMYCES VOLATILIZATION X-RAY DIFFRACTION |