Author/Editor		Kuzman, Drago; Fon Tacer, Klementina; Černe, M; Režen, Tadeja; Ačimovič, Jure; Čegovnik, Urška; Kocjan, Darko; Urleb, Uroš; Rozman, Damjana
Title		Modulation of hepatic transcriptomein the poloxamer P-407 hyperlipidemia mouse model
Type		članek
Source		Acta Chim Slov
Vol. and No.		Letnik 56
Publication year		2009
Volume		str. 262-9
Language		eng
Abstract		The poloxamer P-407 hyperlipidemia mouse is a genetically unaltered, nondiet-induced model of atherosclerosis which is useful in studying effects of lipid lowering drugs. While the model is relatively well described on the biochemical level, the knowledge regarding the global gene expression was lacking so far. As expected, the P-407 elevates plasma triglycerides and cholesterol. Using microarray analysis and RT-PCR we show for the first time that poloxamer triggers a concerted modulation of hepatic genes involved in multiple steps of atherogenesis, such as lipid metabolism, initiation of atherogenesis, lesion formation, and atheroma progression. 106 hepatic genes were upregulated and only 40 downregulated, suggesting that upregulation of atherogenic genes precedes the atheroma formation. Modulated expression of circadian genes has also been observed in this type of hyperlipidemia but further studies are needed to elucidate whether this effects the rhythm of animals.
Descriptors		HYPERLIPIDEMIA ATHEROSCLEROSIS HYPERTRIGLYCERIDEMIA HYPERCHOLESTEROLEMIA POLOXALENE DISEASE MODELS, ANIMAL GENE EXPRESSION POLYMERASE CHAIN REACTION MICE, INBRED C57BL