Author/Editor		van Dijk, Jeroen P; Cankar, Katarina; Scheffer, Stanley J; Beenen, Henriek G; Shepherd, Louise VT; Stewart, Derek; Davies, Howard V; Wilkockson, Steve J; Leifert, Carlo; Gruden, Kristina; Kok, Esther J
Title		Transcriptome analysis of potato tubers - Effects of different agricultural practices
Type		članek
Source		J Agric Food Chem
Vol. and No.		Letnik 57
Publication year		2009
Volume		str. 1612-23
Language		eng
Abstract		The use of profiling techniques such as transcriptomics, proteomics, and metabolomics has been proposed to improve the detection of side effects of plant breeding processes. This paper describes the construction of a food safety-oriented potato cDNA microarray (FSPM). Microarray analysis was performed on a well-defined set of tuber samples of two different potato varieties, grown under different, well-recorded environmental conditions. Data were analyzed to assess the potential of transcriptomics to detect differences in gene expression due to genetic differences or environmental conditions. The most pronounced differences were found between the varieties Sante and Lady Balfour, whereas differences due to growth conditions were less significant. Transcriptomics results were confirmed by quantitative PCR. Furthermore, the bandwidth of natural variation of gene expression was explored to facilitate biological and/or toxicological evaluation in future assessments.
Descriptors		POTATOES DNA, COMPLEMENTARY GENE EXPRESSION TRANSCRIPTION, GENETIC PLANT PROTEINS FOOD ANALYSIS OLIGONUCLEOTIDE PROBES POLYMERASE CHAIN REACTION AGROCHEMICALS ANALYSIS OF VARIANCE