Author/Editor		Groth, Casper; Petersen, Randi Fons; Piškur, Jure
Title		Diversity in organization and the origin of gene orders in the mitochondrial DNA molecules of the genus Saccharomyces
Type		članek
Source		Mol Biol Evol
Vol. and No.		Letnik 17, št. 12
Publication year		2000
Volume		str. 1833-41
Language		eng
Abstract		Sequencing of the Saccharomyces cerevisiae nuclear and mitochondrial genomes provided a new background for studies on the evolution of the genomes. In this study, mitochondrial genomes of a number of Saccharomyces yeasts were mapped by restriction enzyme analysis, the orders of the genes were determined, and two of the genes were sequenced. The genome organization, i.e., the size, presence of intergenic sequences, and gene order, as well as polymorphism within the coding regions, indicate that Saccharomyces mtDNA molecules are dynamic structures and have undergone numerous changes during their evolution. Since the separation and sexual isolation of different yeast lineages, the coding parts have been accumulating point mutations, presumably in a linear manner with the passage of time. However, the accumulation of other changes may not have been a simple function of time. Larger mtDNA molecules belonging to Saccharomyces sensu stricto yeasts have acquired extensive intergenic sequences, including guanosine-cytosine-rich clusters, and apparently have rearranged the gene order at higher rates than smaller mtDNAs belonging to the Saccharomyces sensu lato yeasts. While within the sensu stricto group transposition has been a predominant mechanism for the creation of novel gene orders, the sensu lato yeasts could have used both transposition- and inversion-based mechanisms.
Descriptors		EVOLUTION, MOLECULAR FUNGAL PROTEINS GENOME, FUNGAL BASE SEQUENCE CHROMOSOME MAPPING DNA, MITOCHONDRIAL MOLECULAR SEQUENCE DATA PHYLOGENY POINT MUTATION SACCHAROMYCES SEQUENCE ANALYSIS, DNA