Author/Editor     Naumov, GI; Naumova, ES; Marinoni, G; Piškur, J
Title     Genetic analysis of Saccharomyces castellii, S. exiguus and S. martiniae yeasts
Type     članek
Source     Genetika
Vol. and No.     Letnik 34, št. 4
Publication year     1998
Volume     str. 565-8
Language     eng, rus
Abstract     Genetic lines of three biological yeast species of the Saccharomyces sensu lato group - S. castellii, S. exiguus, and S. martiniae - were created. Homo- and heterothalmic monosporic fertile strains were marked by different auxotrophic mutations. Conditions for the hybridization of the Saccharomyces sensu lato yeast were established. The mating of spores or haploid cells of auxotrophic complementary mutants was performed on the complete YPD medium. Prototrophic diploid hybrids were selected on a minimal medium. In S. castellii, the hybridization of diploid cells was demonstrated. All three species studied were suitable for tetrad analysis.
Descriptors     DNA, FUNGAL
SACCHAROMYCES
SPECIES SPECIFICITY