Author/Editor     Piškur, Jure
Title     The transmission disadvantage of yeast mitochondrial intergenic mutants is eliminated in the mgt1 (cce1) background
Type     članek
Source     J Bacteriol
Vol. and No.     Letnik 179, št. 17
Publication year     1997
Volume     str. 5614-7
Language     eng
Abstract     A trans-acting element, MGT1 (also called CCE1), has previously been shown to be required in Saccharomyces cerevisiae for the preferential transmission of petite mitochondrial DNA (mtDNA) molecules over wild-type mtDNA molecules. In the present study a possible role of this nuclear gene in the transmission of mtDNA from various respiration-competent mutants was studied. Several of these mutants, lacking one or the other of two biologically active mitochondrial intergenic sequences, were employed in genetic crosses. When these deletion mutants were crossed to the parental wild-type strain in the MGT1/CCE1 background, the progeny contained predominantly wild-type mtDNA molecules. When crosses were performed in the mgt1/cce1 background, the parental molecules interacted in zygotes and underwent homologous recombination but wild-type and intergenic-deletion alleles were transmitted with equal frequencies.
Descriptors     MITOCHONDRIA
CROSSES, GENETIC
DNA, FUNGAL
DNA, MITOCHONDRIAL
ENDODEOXYRIBONUCLEASES
MUTATION
RECOMBINATION, GENETIC
RESTRICTION MAPPING
SACCHAROMYCES CEREVISIAE