Author/Editor		Piškur, Jure
Title		Respiratory-competent yeast mitochondrial DNAs generated by deleting intergenic regions
Type		članek
Source		Gene
Vol. and No.		Letnik 81, št. 1
Publication year		1989
Volume		str. 165-8
Language		eng
Abstract		Two respiratory-competent yeast strains having mitochondrial (mt) DNA characterized by single non-overlapping deletions, encompassing intergenic sequences, have been crossed. Diploid daughter clones have been screened by electrophoresis of mtDNA fragments, and a respiratory-competent clone (ER8.75), having a recombinant small mtDNA with both parental deletions, has been detected. ER8.75 mtDNA lacks around 20% of wild-type intergenic sequences, encompassing three ori/rep sequences. This mutant could be helpful in analyzing the organelle genome and, particularly, the function of intergenic sequences.
Descriptors		CHROMOSOME DELETION CROSSES, GENETIC DNA RESTRICTION ENZYMES DNA, MITOCHONDRIAL SACCHAROMYCES CEREVISIAE