Author/Editor		Gabrijel, Mateja; Bergant, Martina; Kreft, Marko; Jeras, Matjaž; Zorec, Robert
Title		Fused late endocytic compartments and immunostimulatory capacity of dendritic-tumor cell hybridomas
Type		članek
Source		J Membr Biol
Vol. and No.		Letnik 229, št. 1
Publication year		2009
Volume		str. 11-8
Language		eng
Abstract		Late endocytic compartments, containing MHC class II molecules in antigen presenting cells, fuse to each other in order to deliver antigens to these molecules. We have shown previously that fusion of late endocytic compartments takes place also in hybridomas. Therefore, we investigate here whether the level of fused late endocytic compartments affects the immunostimulatory capacity of hybridomas obtained by the electrofusion of dendritic and tumor cells. The level of fused late endocytic compartments in a single hybridoma cell was assessed and samples of electrofused cells were then cocultured with autologous T cells, resulting in the priming of naive T cells. To test the immunostimulatory capacity of hybridoma cells, T-cell-induced cytotoxicity of tumor cells was assayed. The results demonstrate that in vitro cytotoxic T cell responses are enhanced if a higher percentage of fused late endocytic compartments is present in the cell population of electrofused hybridoma cells.
Descriptors		HYBRIDOMAS DENDRITIC CELLS CACO-2 CELLS ENDOCYTOSIS IMMUNIZATION T-LYMPHOCYTES, CYTOTOXIC IMMUNOHISTOCHEMISTRY MICROSCOPY, CONFOCAL