Author/Editor		Zorn, Branko; Virant-Klun, Irma; Drobnič, Sašo; Šinkovec, Jasna; Meden-Vrtovec, Helena
Title		Male and female factors that influence ICSI outcome in azoospermia or aspermia
Type		članek
Source		Reprod Biomed Online
Vol. and No.		Letnik 18, št. 2
Publication year		2009
Volume		str. 168-76
Language		eng
Abstract		Factors that influenced the clinical results of 220 first-attempt intracytoplasmic sperm injection (ICSI) cycles with testicular spermatozoa were evaluated in 107 men with non-obstructive azoospermia, 72 with obstructive azoospermia and 41 with aspermia. Linear and logistic regression analysis showed that the fertilization rate depended positively on Johnsen score (P = 0.016) and on the type of ovarian stimulation: a higher fertilization rate was observed after ovarian stimulation with agonist and recombinant FSH than after stimulation with agonist and urinary menopausal gonadotrophin (P = 0.026). Embryo development to the blastocyst stage was predicted positively by the number of injected oocytes (P = 0.016) and negatively by male FSH concentration (P = 0.019). A higher proportion of blastocysts developed after the use of frozen-thawed spermatozoa in comparison to fresh spermatozoa (P = 0.034). Embryo development to the blastocyst stage influenced pregnancy (P = 0.002) and live birth outcomes (P = 0.005); live birth was also predicted by female age (P = 0.046). Embryo culture to day 5 in comparison to day 2 did not provide higher live birth rates. In azoospermia/aspermia, the ICSI outcome depends on both male factors (FSH, Johnsen score, sperm status and motility) and female factors (age, number of injected oocytes).
Descriptors		ADULT PREGNANCY PREGNANCY RATE PROGNOSIS RETROSPECTIVE STUDIES SEX FACTORS TREATMENT OUTCOME