Author/Editor		Glinšek, Urška; Udovič, Tina; Cerar, Tjaša; Strle, Franc; Ružić-Sabljić, Eva
Title		Protein profile determination of Borrelia afzelii and Borrelia garinii isolated from skin and cerebrospinal fluid
Type		članek
Source		World J Microb Biot
Vol. and No.		Letnik 25
Publication year		2009
Volume		str. 1287-96
Language		eng
Abstract		In Europe the initial skin manifestation of Lyme borreliosis (erythema migrans) is predominantly caused by Borrelia afzelii while nervous system involvement is usually associated with Borrelia garinii. The aim of our study was to compare protein profiles of B. afzelii and B. garinii isolated from skin of patients with erythema migrans and from cerebrospinal fluid (CSF) of patients with Lyme neuroborreliosis. We analyzed 187 Borrelia strains, 74 B. afzelii and 113 B. garinii, for the presence of flagellin, and outer surface proteins A, B and C. Their protein profiles were obtained by SDS‐PAGE and analyzed by computer program Gel Doc. Differences in the presence of proteins were found comparing isolates according to species (B. afzelii and B. garinii) and to their origin (skin and CSF isolates); the heterogeneity regarding the presence or absence of borrelial proteins was established within particular species. Protein profiles of the analyzed strains showed differences in the number, amount and molecular mass of analyzed proteins. Distinctions in the synthesis of outer surface proteins may play a role in the dispersal of borreliae within and between animal reservoir and vector ticks, as well as in pathogenesis of Lyme borreliosis in humans.
Descriptors		LYME DISEASE BORRELIA BACTERIAL PROTEINS FLAGELLIN BACTERIAL OUTER MEMBRANE PROTEINS ERYTHEMA CHRONICUM MIGRANS CEREBROSPINAL FLUID ELECTROPHORESIS, POLYACRYLAMIDE GEL