Author/Editor		Erman, Andreja; Zupančič, Daša; Jezernik, Kristjan
Title		Apoptosis and desquamation of urothelial cells in tissue remodeling during rat postnatal development
Type		članek
Source		J Histochem Cytochem
Vol. and No.		Letnik 57, št. 8
Publication year		2009
Volume		str. 721-30
Language		eng
Abstract		Postnatal rat urothelium was studied from day 0 to day 14, when intense cell loss as part of tissue remodeling was expected. The morphological and biochemical characteristics of urothelial cells in the tissue and released cells were investigated by light and electron microscopy, by terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL) assay, by annexin V/propidium iodide assay, and by immunofluorescent detection of active caspases and tight-junction protein occludin. Intense apoptosis and massive desquamation were detected between postnatal days 7 and 10. During this period, active caspases and TUNEL-positive cells were found in the urothelium. Disassembled cell-cell junctions were detected between cells. The majority of desquamated cells expressed no apoptotic cell morphology, but were active caspase positive and TUNEL positive. Ann+/PI- apoptotic bodies and desquamated Ann+/PI+ cells were detected in the lumen. These results indicate that apoptosis and desquamation participate in urothelial cell loss in the rat early postnatal period, indispensable for fast urothelial remodeling during development.
Descriptors		UROTHELIUM APOPTOSIS FLUORESCENT ANTIBODY TECHNIQUE DNA FRAGMENTATION ANNEXIN V RATS, WISTAR PROPIDIUM IODINE MICROSCOPY, ELECTRON BLOTTING, WESTERN