Author/Editor		Milisav, Irina; Nipič, Damijan; Šuput, Dušan
Title		The riddle of mitochondrial caspase-3 from liver
Type		članek
Source		Apoptosis
Vol. and No.		Letnik 14, št. 9
Publication year		2009
Volume		str. 1070-5
Language		eng
Abstract		Caspase-3 is one of the main executors of apoptosis. Its zymogen procaspase-3 was localized to cytosol, mitochondria and nuclei. The subcellular location of procaspase-3 in liver was reported by several studies to be either cytosolic or cytosolic and mitochondrial. Our aim was to investigate these separate procaspase-3 pools to differentiate the pathways of their activation. By cell fractionation, immunocytochemistry, and confocal microscopy we report that there is a single procaspase-3 pool located to the cytosol in primary hepatocytes and in fractions of rat liver. In contrast, it depends on the isolation purity whether procaspase-3 is located in mitochondria of non-parenchymal liver cells, or not. All preparations with mitochondrial procaspase-3 fractions contain traces of haemoglobin, indicating the presence of some erythrocytes, which are the source of mitochondrial procaspase-3. Since erythrocytes migrate with mitochondria in subcellular fractionations, it is important to check for haemoglobin, before localizing the protein to mitochondria.
Descriptors		MITOCHONDRIA, LIVER APOPTOSIS CYTOSOL CELL FRACTIONATION IMMUNOHISTOCHEMISTRY MICROSCOPY, CONFOCAL HEMOGLOBINS ERYTHROCYTES CELLS, CULTURED