| Author/Editor | Homan, Matjaž; Luzar, Boštjan; Kocjan, Boštjan J; Orel, Rok; Močilnik, Tina; Shrestha, Maja; Kveder, Maja; Poljak, Mario | |
| Title | Prevalence and clinical relevance of cagA, vacA, and iceA genotypes of Helicobacter pylori isolated from Slovenian children | |
| Type | članek | |
| Source | J Pediatr Gastroenterol Nutr | |
| Vol. and No. | Letnik 49, št. 3 | |
| Publication year | 2009 | |
| Volume | str. 289-96 | |
| Language | eng | |
| Abstract | Background: Although infection with Helicobacter pylori in children mostly induces asymptomatic chronic gastritis, the clinical outcome of H pylori infection is generally unpredictable. To identify the risk subgroup of infected children who can progress toward serious gastrointestinal disease, we assessed the prevalence of H pylori virulence genes cagA, vacA, and iceA in children from southeastern Europe and correlated their presence with the severity of histological changes in the stomach. Materials and methods: A total of 165 children (age range 4-18 years, mean 13 years) with H pylori infection were studied for a 6-year period. Virulence genes were determined by polymerase chain reaction from biopsy samples. Results: The cagA gene was present in 61.2% of patients. The predominant vacA genotype was s1m1 (42%), followed by s1m2 (28%), and s2m2 (24%). IceA genotypes iceA1 and iceA2 were detected in 62% and 31% of the samples, respectively. Multiple genotypes were found in 11.5% of isolates. The H pylori density score, the degree of chronic and acute inflammation, correlated with a cagA-positive status (P < 0.01, P < 0.01, P = 0.01, respectively). Higher bacterial infiltration (P < 0.01) and degree of chronic inflammation (P = 0.03) were detected in vacA s1-positive samples. Conclusion: CagA, vacA s1m1, and iceA1 genotypes are the predominant genotypes of H pylori isolated from the southeastern European pediatric population. CagA and vacA s1 are important virulence determinants of H pylori in children, but were not found associated with an increased incidence of precancerous gastric lesions. | |
| Descriptors | HELICOBACTER INFECTIONS HELICOBACTER PYLORI GENOTYPE GASTRITIS METAPLASIA CHILD |