Author/Editor     Chao, Steven; Benowitz, Larry I; Krainc, Dimitri; Irwin, Nina
Title     Use of a two-hybrid system to investigate molecular interactions of GAP-43
Type     članek
Source     Brain Res Mol Brain Res
Vol. and No.     Letnik 40, št. 2
Publication year     1996
Volume     str. 195-202
Language     eng
Abstract     We used the 'interaction trap' (two-hybrid system) to identify polypeptides that interact with the neuronal phosphoprotein, GAP-43, in an intracellular environment. GAP-43 (neuromodulin, B-50, F1), a protein kinase C (PKC) substrate important for the growth and plasticity of neuronal connections, has been implicated in vitro in several signal transduction pathways. In the yeast-based cloning system, the only strong interaction that was detected between GAP-43 and the calcium effector protein, calmodulin (CaM). PKC phosphorylates GAP-43 on serine 41. When we changed this serine to an aspartate residue to mimic constitutive phosphorylation, the interaction with CaM was blocked. Surprisingly, the N-terminal third of GAP-43 alone bound CaM more strongly than did intact GAP-43, suggesting that the protein's C-terminus may play a role in modulating the interaction with CaM. These results, along with other recent findings, suggest a novel role for the interaction between GAP-43 and CaM.
Descriptors     CALMODULIN
CELL MEMBRANE
CHROMOSOME MAPPING
MEMBRANE GLYCOPROTEINS
MUTATION
NERVE TISSUE PROTEINS
PLASMIDS