Author/Editor		Sikdar, SK; Zorec, R; Mason, WT
Title		cAMP directly facilitates Ca-induced exocytosis in bovine lactotrophs
Type		članek
Source		FEBS Lett
Vol. and No.		Letnik 273, št. 1-2
Publication year		1990
Volume		str. 150-4
Language		eng
Abstract		We have used the whole cell patch clamp technique on single prolactin-secreting bovine lactotrophs to measure plasma membrane capacitance (Cm), an index of membrane surface area, under voltage-clamp during cytosol dialysis with Ca and cAMP. cAMP increased the magnitude and rate of Ca-induced exocytosis (Cm increase) without affecting membrane conductance; however, cAMP had no detectable effect on Cm when intracellular Ca was low. We thus report new evidence that cAMP can facilitate Ca-induced secretion in a synergistic fashion, by acting directly on the secretory apparatus, independently of membrane conductance activation.
Descriptors		CALCIUM CYCLIC AMP EXOCYTOSIS PITUITARY GLAND, ANTERIOR PROLACTIN CALCIUM CATTLE CELL MEMBRANE CELLS, CULTURED EGTAZIC ACID ELECTRIC CONDUCTIVITY KINETICS MEMBRANE POTENTIALS PITUITARY GLAND, ANTERIOR