Author/Editor     Bavec, Aljoša; Ličar, Alenka
Title     Functional characterization of N-terminally GFP-tagged GLP-1 receptor
Type     članek
Source     Journal of Biomedicine and Biotechnology (Online)
Vol. and No.     , št. 498149
Publication year     2009
Volume     str. 1-10
Language     eng
Abstract     The glucagon-like peptide-l receptor (GLP-l receptor) mediates important effects on peripheral tissues and the central nervous system. It seems one of the most promising therapeutic targets for treatment of diabetes mellitus type 2. Surprisingly, very little is known about the cellular mechanisms that regulate its function in vivo. One of the approaches to study receptor dynamics, expression, or signaling is using GFP-tagged fluorescent proteins. In this study, we synthesized and characterized N-terminally GFP-tagged GLP-l (GFP-GLP-l) receptor in eHO cells. We demonstrated that GFP-GLP-l receptor is weakly expressed in the plasma membranes and is functionally coupled to adenylyl cyclase via heterotrimeric G-proteins, similarly as its wild type.
Descriptors     CHO CELLS
RECEPTORS, GLUCAGON
PEPTIDES
ADENYL CYCLASE
RECOMBINANT PROTEINS
PROTEIN BINDING
BINDING SITES
CYCLIC AMP
MICROSCOPY, CONFOCAL
IMMUNOBLOTTING
DIABETES MELLITUS, NON-INSULIN-DEPENDENT