Author/Editor     Kastelic, D; Frković-Grazio, S; Baty, D; Truan, G; Komel, R; Pompon, D
Title     A single-step procedure of recombinant library construction for the selection of efficiently produced llama VH binders directed against cancer markers
Type     članek
Source     J Immunol Methods
Vol. and No.     Letnik 350, št. 1-2
Publication year     2009
Volume     str. 54-62
Language     eng
Abstract     Heavy chain antibodies are naturally occurring in camelidae (camels and llamas). Their variable domain (VHH) can be efficiently produced as a recombinant protein in E. coli with a large range of applications in the fields of diagnostics and immunotherapy. Standard cloning approach involves resolution of VHH from the heavy chain variable domain of conventional antibodies (VH) by a nested PCR amplification followed by a phage display based selection. Present work illustrates that in contrast to usual finding, specific, good affinity and efficiently expressed VH domain of conventional antibodies can be selected from the co-amplification products of VH and VHH cDNAs. Sequence analysis illustrated that following the two first rounds of selection against cancer markers, similar number of VH and VHH binders were observed. However, after a third round, the more specific binders directed against p53, VEGF, BCL-2 proteins surprisingly contain only VH specific hallmarks. Characterisation of the specificity, affinity and productivity of selected VH binders is described. Because llama VHs show higher sequence and structural homology with the human VH III group than llama VHHs (Vu et al., 1997), they constitute very interesting agents in therapeutic applications, especially in human immunotherapy and cancer treatment.
Descriptors     TUMOR MARKERS, BIOLOGICAL
ANTIBODY FORMATION
ANTIBODY SPECIFICITY
IGG
RECOMBINATION, GENETIC
RECOMBINANT PROTEINS
TRANSFECTION
ESCHERICHIA COLI
COLIPASES
CAMELIDS, NEW WORLD