| Author/Editor | Strojnik, Tadej; Kavalar, Rajko; Zajc, Irena; Diamandis, Eleftherios P; Oikonomopoulou, Katerina; Lah Turnšek, Tamara | |
| Title | Prognostic impact of CD68 and kallikrein 6 in human glioma | |
| Type | članek | |
| Source | Anticancer Res | |
| Vol. and No. | Letnik 29, št. 8 | |
| Publication year | 2009 | |
| Volume | str. 3269-79 | |
| Language | eng | |
| Abstract | Aims: To evaluate the expression of CD68 and kallikrein 6 in human gliomas, and investigate their prognostic significance for survival of brain cancer patients in comparison to some known prognostic markers. Patients and methods: Histological sections of 51 primary astrocytic tumours (11 benign, 40 malignant) were immunohistochemically stained for CD68, cathepsin B, kallikrein 6 and Ki-67. CD68 and kallikrein 6 expressions were also analyzed by real-time PCR in nine brain tumour biopsies. Results: Both microglia and tumour cells expressed CD68. High CD68 and cathepsin B staining scores were significantly, more frequent in the malignant than in the benign tumours (p=0.036 and p=0.014, respectively). In contrast, the benign group presented a stronger immunoreactivity for kallikrein 6 compared with the malignant tumours (p=0.013). A CD68 staining score of tumour cells higher than 3 was a significant predictor of shorter overall survival (p<0.01) in all patients and of borderline significance in the malignant group (p=0.057). Strong CD68 staining was of greater predictive value in the subgroup of anaplastic astrocytomas (p=0.021). Furthermore, as expected on the basis of our previous studies, prognostic significance was confirmed for cathepsin B, but not for any of the other markers under evaluation. Conclusion: Kallikrein 6 was down-regulated in malignant glioma, but this differential expression did not have an impact on patient prognosis. In contrast, immunostaining of glioma tissue for CD68 and for cathepsin B may be used for prognosis of survival in these patients. This finding suggests that besides the known role of cathepsin B in invasion and angiogenesis, CD68 may be also associated with glioma progression. | |
| Descriptors | CATHEPSIN B MICROGLIA BRAIN NEOPLASMS PROGNOSIS GLIOMA IMMUNOHISTOCHEMISTRY |