Author/Editor		Maretić, Tomislav; Benić, Branka; Ðaković-Rode, Oktavija; Beritić, Dubravka; Ružić-Sabljić, Eva
Title		Prva izolacija borelije (Borrelia afzelii) iz likvora bolesnika s neuroboreliozom u Hrvatskoj
Translated title		First isolation of Borellia sp. (Borrelia afzelii) from cerebrospinal fluid in a patient with neuroborreliosis Croatia
Type		članek
Source		Infektol Glas
Vol. and No.		Letnik 29, št. 2
Publication year		2009
Volume		str. 65-70
Language		cro
Abstract		We describe a patient with lyme borreliosis that manifested with facial erythema migrans one mo nth after tick bite and consequently with peripheral facial paresis with lymphocyte meningitis (127 cells/μL and 78 % lymphocytes). Clinical picture ofneuroborreliosis was relatively mild. The patient received ceftriaxone parenteral1y for three weeks, with beneficial effect of antibiotic therapy and aImost complete resolution of facial paresis untiI discharge from hospital.Reactive IgG anti bodi es to B. burgdorferi were found in the patient's serum. A positive index of intrathecal synthesis ofIgM and [gG anti bodi es to borreliae was determined (specific test IDEIA Lyme Neuroborreliosis Oxoid, Great Britain). Immunological examination for detection oftick-borne meningoencephalitis, varicel1a-zoster virus and herpes simplex proved negative in both serum and cerebrospinal f1uid (CSF). B. burgdoljeri sensu lato was isolated from CSF using specific culturing techniques at the Institute ofMicrobiology and Immunology of the Medical Faculty, University of Ljubljana. After cultivation of the pathogen, identification of Borrelia species was performed by using restriction enzyme Miti/ thus detennining the causative agent of neuroborreliosis: Borrelia afzelii. Although neuroborreliosis has been diagnosed andAreated for over twenty years in Croatia, so far Bon"elia has never been isolated from cerebrospinal f1uid in our patients.
Descriptors		LYME DISEASE BORRELIA NERVOUS SYSTEM DISEASES IGG IGM CHILD CROATIA