Author/Editor		Jančar, N; Kocjan, BJ; Poljak, M; Vrtačnik-Bokal, E
Title		Comparison of paired cervical scrape and tumor tissue samples for detection of human papillomaviruses in patients with cervical cancer
Type		članek
Source		Eur J Gynaecol Oncol
Vol. and No.		Letnik 30, št. 6
Publication year		2009
Volume		str. 675-8
Language		eng
Abstract		Purpose of im'estigation: To compare the detection and distribution of HPV genotypes in paired cervical scrape samples and tumor tissue samples in patients with cervical cancer. Methods: Forty cervical scrape samples and 40 paired archival or fresh frozen tissue sample s were collected from women with cervicaJ cancer. Polymerase chain reaction with GP5+ and GP6+ primers was performed in all sampJes for HPV DKA detection. All GP5+/GP6+ negative samples were additionally tested using INNO-LiPA HPV Genotyping Extra Test. Results: Overall, 39/40 (97.5%) of CC samples were HPV DNA positive. HPV 16 was found in 24/40 samples, HPV 18 in 5/40 samples. A co-infection with two different HPV genotypes was identified in one cervical scrape speci men, while in tissue samples only single infections were detected. Overall agreement between paired samples was 98.75%. Conc/usion: The present study has shown that cervical scrape samples are equally useful for HPV genotype determination as tumor tissue samples in patients with cervical cancer. They can be used as accurate clinical sampJes for detection of HPV genotype causing cervi cal cancer or for epidemiological molecular studies.
Descriptors		CERVIX NEOPLASMS PAPILLOMAVIRUS, HUMAN GENOTYPE NEOPLASM STAGING