Author/Editor		Kovačič, Borut
Title		Izbira spermija v IVF laboratoriju
Translated title		Selection of sperm in the IVF laboratory
Type		članek
Source		In: Zorn B, Vrtačnik-Bokal E, editors. Premagovanje moške neplodnosti: napredek in novi izzivi. Ljubljana: Slovensko društvo za reproduktivno medicino,
Publication year		2009
Volume		str. 22-30, 103-104
Language		slo
Abstract		Laboratory semen preparation and sperm selection have an important role in infertility treatment by in vitro fertilization (IVF) methods. Sperm selection influences the fertilization rate, embryo development and success rates of the treatment. The aim of this paper is to review the numerous laboratory methods, by which the optimal sperm can be isolated from the semen. The advantages and drawbacks of the swim-up technique - the most frequently used sperm washing method in classical IVT - are described. Numerous modifications, which have developed from the swim-up method are decribed as well. They are suitable for preparation of oligoastheno- or cryptozoospermic semen samples. The techniques for easiest searching and identification of viable sperm in the samples with sperm motility problem are shown too. These are the samples with total sperm immotility and testicular sperm samples. The developments of optical technology and biotechnology have increased the possibilities for the selection of the morphologically best sperm and those with undamaged DNA.
Summary		Pri zdravljenju neplodnosti z zunajtelesno oploditvijo imata laboratorijska priprava semena in izbira semenčic za oploditev jajčnih celic zelo pomembno vlogo. Izbor semenčic vpliva na stopnjo oplojenosti, embrionalni razvoj in uspešnost postopka zdravljenja. Namen prispevka je prikazati številne laboratorijske metode, s katerimi se je iz semenskega izliva poskušalo izolirati najoptimalnejše semenčice. Opisane so prednosti in slabosti swim-up metode, ki jo najpogosteje uporabljamo pri klasični oploditvi in vitro. Naštete so tudi številne različice, ki so se sčasoma razvile iz te osnovne metode in so primerne v posebnih primerih, kot so težka oligoastenoterato- in kriptozoospermija. Opisane so tehnike za lažje iskanje in prepoznavanje živih semenčic iz vzorcev semena, kjer je gibljivost najbolj problematična. To so vzorci s popolnoma negibljivimi semenčicami in vzorci tkiva mod. Razvoj optične tehnologije je izboljšal možnosti izbora morfološko najoptimalnejših semenčic in tistih z nepoškodovano DNK.
Descriptors		INFERTILITY, MALE FERTILIZATION IN VITRO SPERM MOTILITY