Author/Editor		Vardjan, Nina; Stenovec, Matjaž; Jorgačevski, Jernej; Kreft, Marko; Zorec, Robert
Title		Mehanizmi eksocitoze
Translated title		Mechanisms of exocytosis
Type		članek
Source		Acta Biol Slov
Vol. and No.		Letnik 52, št. 2
Publication year		2009
Volume		str. 95-106
Language		eng
Abstract		Vesicles are cellular organelles, in which signaling mole cuI es (neuwtransmitters or hormone s) are stored and are essential for the function ofneurons and endocrine cells in supporting the communication between tissues and organs. Upon stimulation the signaling molecules stored inside vesicles are released from cells by exocytosis. This fundamental biological process consists of membrane fusion between the vesicles and the plasma membrane, leading to the formation of an aqueous channel- the fusion pore - through which signaling molecules exit into the extracellular space or blood stream. The vesicle cargo discharge initially requires the delivery of vesicles to the plasma membrane, where vesicles dock and get primed for fusion with the plasma membrane. Classical view holds that stimulation initiates the fusion pore formation and vesicle cargo discharge in an all-or-none fashion. ance formed the fusion pore may close (transient, kiss-and-run exocytosis) or expand, leading to the full collapse of the vesicle membrane into the plasma membrane (full fusion exocytosis). However, recent studies indicate that exocytosis may not be as simple. Here we highlight the novel findings which indicate that transient fusion pore is subject to regulations, which affect the release competence of a single vesicle. Our recent studies have shown that in pituitary lactotrophs vesicle release of peptide signaling molecules involves modulation of fusion pore kinetics and fusion pore conductance.
Descriptors		EXOCYTOSIS ORGANELLES PITUITARY GLAND PROLACTIN MEMBRANE FUSION BIOLOGICAL TRANSPORT, ACTIVE SIGNAL TRANSDUCTION MICROSCOPY, ELECTRON, SCANNING MICROSCOPY, CONFOCAL RATS