Author/Editor		Šarc, Lucija; Lipnik-Štangelj, Metoda
Title		Comparison of ethanol and acetaldehyde toxicity in rat astrocytes in primary culture
Type		članek
Source		Arh Hig Rada Toksikol
Vol. and No.		Letnik 60, št. 3
Publication year		2009
Volume		str. 297-305
Language		eng
Abstract		This study compared the effects oftoxicity of ethanol and its first metabolite acetaldehyde in rat astrocytes through celi viability and celi proliferation. The cells were treated with different concentrations of ethanol in the presence or absence of a catalase inhibitor 2-amino-l ,2,4 triazole (AMT) or with different concentrations of acetaldehyde. Celi viability was assessed using the trypan blue test. Celi proliferation was assessed after 24 hours and after seven day s of exposure to either ethanol or acetaldehyde.We showed that both ethanol and acetaldeliyde decreased celi viability in a dose-dependent manner. In proliferation studies, after seven days of exposure to either ethanol or acetaldehyde, we observed a significant dose-dependent decrease in celi number. The protein content study showed biphasic dose-response curves, after 24 ho urs and seven days of exposure to either ethanol or acetaldehyde. Co-incubation in the presence of AMT significantly reduced the inhibitory effect of ethanol on celi proliferation.We conc1uded that long-term exposure of astrocytes to ethanol is more toxic than acute exposure. Acetaldehyde is a much more potent toxin than ethanol, and at least a part of ethanol toxicity is due to ethanol's first metabolite acetaldehyde.
Descriptors		ASTROCYTES ALCOHOL, ETHYL ACETALDEHYDE CELL SURVIVAL TRIAZOLES CELL DIVISION TRYPAN BLUE RATS, WISTAR