Author/Editor		Grohovaz, F; Lorenzon, P; Ruzzier, F; Zorec, R
Title		Properties of acetylcholine receptors in adult rat skeletal muscle fibers in culture
Type		članek
Source		J Membr Biol
Vol. and No.		Letnik 136, št. 1
Publication year		1993
Volume		str. 31-42
Language		eng
Abstract		The distribution and biophysical properties of acetylcholine receptors were studied, using morphological and patch-clamp techniques, in adult rat skeletal muscle fibers dissociated by collagenase and maintained in culture. Up to ten hours after dissociation, there were no changes in either the distribution or the biophysical properties ofjunctional acetylcholine receptors. In long-term culture (5 to 14 days), a new type of acetylcholine receptor was inserted all over the muscle fibers; the channel properties were characterized by a longer open time and a smaller conductance, similar to what has been observed in in vivo denervated muscles. Using autoradiography, we found that during culture an irnpaired incorporation of new acetylcholine receptors in the former endplates caused a progressive decrease in the density ofjunctional acetylcholine receptors. This contrasts with muscle fibers denervated in vivo, where the density of receptors does not change after denervation. Key words: Acetylcholine channels - Denervation - Patch clamp - Videoimaging
Descriptors		RECEPTORS, CHOLINERGIC MUSCLES RATS IMMUNOHISTOCHEMISTRY MICROSCOPY, ELECTRON RATS, WISTAR MUSCLE DENERVATION MOTOR ENDPLATE TIME FACTORS