Author/Editor     Todorović, Vesna; Serša, Gregor; Mlakar, Vid; Glavač, Damjanž; Flisar, Karel; Čemažar, Maja
Title     Metastatic potential of melanoma cells is not affected by electrochemotherapy
Type     članek
Source     Melanoma Res
Vol. and No.     Letnik 21, št. 3
Publication year     2011
Volume     str. 196-205
Language     eng
Abstract     Electrochemotherapy is a local treatment combining chemotherapy and application of electric pulses to the tumour. Electrochemotherapy with bleomycin and cisplatin has shown its effectiveness in controlling local tumour growth in the treatment of malignant melanoma. However, the effect of electrochemotherapy on the metastatic potential of tumour cells is not known. Prevention of metastasis is an important aspect of successful treatment; however, it is known that metastasis can be induced by different treatment modalities. Therefore, the aim of this study was to evaluate the effect of electrochemotherapy with cisplatin on the metastatic potential of human malignant melanoma cells. Cells treated by electrochemotherapy with cisplatin were tested for their ability to migrate and invade through Matrigel-coated porous membrane. In addition, RNA was isolated from cells after treatment and differentially expressed genes were investigated by microarray analysis to evaluate the effect of electrochemotherapy with cisplatin on gene expression. There were no significant changes observed in cell migration and invasion of melanoma cells after electrochemotherapy. In addition, there were no changes observed in cell adhesion on Matrigel. Gene expression analysis showed that a very low number of genes were differentially expressed after electrochemotherapy with cisplatin. Two genes, LAMB3 and CD63 involved in cell migration, were both downregulated after electrochemotherapy with cisplatin and the expression of metastasis promoting genes was not increased after electrochemotherapy. Our data suggest that electrochemotherapy does not increase the metastatic behaviour of human melanoma cells.
Descriptors     MELANOMA
BLEOMYCIN
CISPLATIN
NEOPLASM METASTASIS
GENE EXPRESSION REGULATION, NEOPLASTIC
OLIGONUCLEOTIDE PROBES
ELECTROPORATION
TUMOR CELLS, CULTURED
NEOPLASM INVASIVENESS