Author/Editor     Cerar, T; Ogrinc, K; Strle, F; Ružić-Sabljić, E
Title     Humoral immune response in Slovenian patients with Lyme neuroborreliosis
Type     članek
Source     Clin Vaccine Immunol
Vol. and No.     Letnik 17, št. 4
Publication year     2010
Volume     str. 645-50
Language     eng
Abstract     The aim of the study was to analyze and compare the humoral immune response in serum and cerebrospinal fluid in 34 adult patients with clinically evident Lyme neuroborreliosis, 27 patients with clinically suspected Lyme neuroborreliosis and 32 patients with tick-borne encephalitis. Additionally, we wanted to compare the findings of two methods for detection of intrathecally synthesized borrelial antibodies: the IDEIA(TM) Lyme neuroborreliosis test using flagellar antigen, and an approach based on the indirect chemiluminiscence immunoassay LIAISON(R) using OspC and VlsE antigens. Borrelial IgM and IgG antibodies in sera of patients with clinically evident Lyme neuroborreliosis were detected with at least one of the methods in 22/34 (64.7%) and 28/34 (82.4%) patients, respectively; the corresponding results in cerebrospinal fluid were 22/34 (64.7%) and 20/34 (58.8%) patients. Intrathecal synthesis of borrelial IgM and/or IgG was found in 19/34 (55.9%) patients: IgM in 17/34 (50%); IgG in 15/34 (44.1%). Results confirmed previous findings on the relationship between duration of illness and the proportion of patients with detectable humoral immune response. The relatively low proportion of intrathecal synthesis of borrelial antibodies and the high ratio of IgM positivity could be explained with of the short duration of neurological disease as evidenced by reported symptoms (median 10 days). Assessment of the humoral immune response in sera and CSF of patients with early Lyme neuroborreliosis confirmed previous findings on the relationship between duration of illness and the proportion of patients with detectable response.
Descriptors     LYME DISEASE
NERVOUS SYSTEM DISEASES
ENCEPHALITIS, TICK-BORNE
ANTIBODIES, BACTERIAL
IGM
IGA
CHEMILUMINESCENCE
BORRELIA BURGDORFERI
SLOVENIA