Author/Editor		Pfleger, Christian; Meierhoff, Guido; Kolb, Hubert; Battelino, Tadej
Title		Association of T-cell reactivity with beta-cell function in recent onset type 1 diabetes patients
Type		članek
Source		J Autoimmun
Vol. and No.		Letnik 34, št. 2
Publication year		2010
Volume		str. 127-35
Language		eng
Abstract		Objective: The aim of the current study was to investigate whether autoantigen directed T-cell reactivity relates to beta-cell function during the first 78 weeks after diagnosis of type 1 diabetes. Research design and methods: 50 adults and 49 children (mean age 27.3 and 10.9 years respectively) with recent onset type 1 diabetes who participated in a placebo-controlled trial of immune intervention with DiaPep277 were analyzed. Secretion of interferon (IFN)-gamma, interleukin (IL)-5, IL-13 and IL-10 by single peripheral mononuclear cells (PBMC) upon stimulation with islet antigens GAD65, heat shock protein 60 (Hsp60) protein-tyrosine-phosphatase-like-antigen (pIA2) or tetanus toxoid (TT) was determined applying ELISPOT; beta-cell function was evaluated by glucagon stimulated C-peptide. Multivariate regression analysis was applied. Results: In general, number of islet antigen-reactive cells decreased over 78 weeks in both adults and children, whereas reactivity to TT was not reduced. In addition, there was an association between the quality of immune cell responses and beta-cell function. Overall, increased responses by IFN-gamma secreting cells were associated with lower beta-cell function whereas IL-5, IL-13 and IL-10 cytokine responses were positively associated with beta-cell function in adults and children. Essentially, the same results were obtained with three different models of regression analysis. Conclusions: The number of detectable islet-reactive immune cells decreases within 1-2 years after diagnosis of type 1 diabetes. Cytokine production by antigen-specific PBMC reactivity is related to beta-cell function as measured by stimulated C-peptide. Cellular immunity appears to regress soon after disease diagnosis and begin of insulin therapy.
Descriptors		DIABETES MELLITUS, INSULIN-DEPENDENT AUTOANTIGENS T-LYMPHOCYTES ISLETS OF LANGERHANS GLUCAGON INTERFERON TYPE II INTERLEUKIN-5 INTERLEUKIN-13 INTERLEUKIN-10 MONOCYTES HEAT-SHOCK PROTEINS PROTEIN-TYROSINE KINASE TETANUS TOXOID