Author/Editor     Gašperšič, R; Kovačič, U; Glišovič, Š; Coer, A; Skalerič, U
Title     Anti-NGF treatment reduces bone resorption in periodontitis
Type     članek
Source     J Dent Res
Vol. and No.     Letnik 89, št. 5
Publication year     2010
Volume     str. 515-20
Language     eng
Abstract     Periodontitis is characterized by periodontal tissue destruction, including the alveolar bone. One of its critical components is the release of pro-inflammatory neuropeptides from sensory nerve endings innervating the periodontium. Since nerve growth factor (NGF) has been reported to up-regulate neuropeptides in sensory neurons, we hypothesized that it would be increased in ligature-induced periodontitis in rats, and that systemic NGF neutralization would reduce the periodontitis-associated alveolar bone resorption. Real-time PCR analysis disclosed a statistically significant time-dependent up-regulation of NGF mRNA in gingiva during 2 weeks of periodontitis. Interestingly, NGF up-regulation was also detected in the contralateral gingiva. In addition, immunohistochemistry of trigeminal ganglion neurons innervating the gingivomucosa demonstrated increased expression of TrkA receptor for NGF. Systemic anti-NGF treatment during periodontitis significantly reduced interleukin-1beta expression in gingiva and bilateral alveolar bone resorption. This suggests that NGF promotes periodontal inflammation and implicates a possible use of anti-NGF treatment in periodontitis.
Descriptors     PERIODONTITIS
ALVEOLAR BONE LOSS
NERVE GROWTH FACTORS
INTERLEUKIN-1
TRIGEMINAL GANGLION
POLYMERASE CHAIN REACTION
RNA, MESSENGER
IMMUNOHISTOCHEMISTRY
RATS, WISTAR