| Author/Editor | Flego, Veljko; Radojčić-Badovinac, Andjelka; Bulat-Kardum, Ljiljana; Matanić, Dubravka; Crnić-Martinović, Marija; Kapović, Miljenko; Ristić, Smiljana | |
| Title | Primary lung cancer and TNF-alpha gene polymorphisms: a case-control study in a Croatian population | |
| Type | članek | |
| Source | Med Sci Monit | |
| Vol. and No. | Letnik 15, št. 7 | |
| Publication year | 2009 | |
| Volume | str. CR361-5 | |
| Language | eng | |
| Abstract | Background: Tumor necrosis factor-alpha (TNF-alpha) is a multifunctional cytokine involved in the pathogenesis of various inflammatory and malignant diseases. Previous studies investigating the role of the TNF-alpha gene polymorphisms in lung cancer have generated contradictory results. The present study investigated whether the TNF-alpha-308 and TNF-alpha-238 polymorphisms are associated with risk and/or severity of disease in Croatian lung cancer patients. This is the first study in a Caucasian population to analyze the influence of these two polymorphisms on multiple types of lung cancer. Material/Methods: In a case-control study, lung cancer patients (n=230) and appropriate age- and sex-matched controls (n=230) were genotyped by the polymerase chain reaction/restriction fragment length polymorphism method. Allele and genotype frequencies were estimated by gene counting. The chi-squared test was used to compare the observed numbers of different TNF-alpha genotypes for the population with those predicted by Hardy-Weinberg equilibrium. Differences in genotype and allele distributions in the patient and control groups were analyzed for statistical significance using the chi-squared test or Fisher's exact test as appropriate. Results: There were no significant differences in the genotype and allele frequencies for the TNF-alpha-308 and TNF-alpha-238 polymorphisms between lung cancer patients and controls. Furthermore, no association between the genotypes and different stages of lung cancer was detected. Conclusion: This study indicates that the TNF-alpha-308 and TNF-alpha-238 polymorphisms do not influence susceptibility to or severity of lung cancer in a Croatian population. | |
| Descriptors | ADULT AGED AGED, 80 AND OVER CASE-CONTROL STUDIES CROATIA GENE FREQUENCY LUNG NEOPLASMS |