Author/Editor		Kapović, Miljenko; Rukavina, Daniel
Title		Kinetics of lymphoproliferative responses of lymphocytes harvested from the uterine draining lymph nodes during pregnancy in rats
Type		članek
Source		J Reprod Immunol
Vol. and No.		Letnik 20, št. 1
Publication year		1991
Volume		str. 93-101
Language		eng
Abstract		Paraaortic lymph node (PALN) cells were harvested from virgin and pregnant rats bearing syngeneic or allogeneic fetuses at all stages of pregnancy including the pre-implantation period. The specific and non-specific alloreactivity of these cells was analyzed in mixed lymphocyte reactivity (MLR) against mitomycin-C treated-paternal strain or unrelated cells. Mitogen stimulation of the cell utilized PHA, Con-A and PWM. Cells bearing T cell markers were labeled in an indirect assay using the monoclonal antibodies W3/25 and MRC OX 8. Specific alloreactivity is strongly suppressed in the pre-implantation and implantation stages of pregnancy. Specific and non-specific alloreactivities were enhanced at mid-pregnancy and normalized by the end of pregnancy. Reactivity to polyclonal mitogens is enhanced at mid-pregnancy, and the CD4+/CD8+ ratio is very low during all phases of pregnancy.
Descriptors		ANIMALS ANTIGEN-PRESENTING CELLS CELL DIVISION CELLS, CULTURED CONCANAVALIN A IMMUNE TOLERANCE LYMPH NODES LYMPHOCYTES PHYTOHEMAGGLUTININS PREGNANCY PREGNANCY, ANIMAL RATS RATS, INBRED STRAINS