Author/Editor | Kapović, Miljenko; Rukavina, Daniel | |
Title | Reactivity to alloantigens and polyclonal mitogens and CD4+/CD8+ cell ratio shifts of cervical lymph node and spleen cells during pregnancy in rats | |
Type | članek | |
Source | J Reprod Immunol | |
Vol. and No. | Letnik 20, št. 2 | |
Publication year | 1991 | |
Volume | str. 165-74 | |
Language | eng | |
Abstract | Cervical lymph node (CLN) cells and spleen cells were harvested from virgin and pregnant rats bearing syngeneic or allogeneic fetuses at all stages of pregnancy including the pre-implantation period. The specific and non-specific alloreactivity of these cells were analyzed in MLR against mitomycin-C treated paternal strain or unrelated cells. Mitogen stimulation of the cell cultures utilized PHA, Con-A and PWM. Cells bearing T cell markers were labeled in an indirect assay using the monoclonal antibodies W3/25 and MRC OX 8. Specific alloreactivity is enhanced at mid-pregnancy in both cell populations. Non-specific alloreactivity was suppressed in the cervical lymph node cells. Spleen cells demonstrated an increased non-specific alloreactivity and T polyclonal mitogen reactivity (PHA and Con-A) at mid-pregnancy. Reactivity to Con-A was depressed in the early phase and at the end of allogeneic pregnancy in the CLN. The CD4+/CD8+ ratio was very low during all phases of pregnancy. | |
Descriptors | ANIMALS ANTIGENS, CD4 ANTIGENS, CD8 CELL DIVISION CERVIX UTERI CONCANAVALIN A IMMUNE TOLERANCE ISOANTIGENS LYMPH NODES LYMPHOCYTE CULTURE TEST, MIXED PHYTOHEMAGGLUTININS POKEWEED MITOGENS PREGNANCY PREGNANCY, ANIMAL RATS RATS, INBRED STRAINS SPLEEN T-LYMPHOCYTES TIME FACTORS |