Author/Editor     Kapović, Miljenko; Rukavina, Daniel
Title     Reactivity to alloantigens and polyclonal mitogens and CD4+/CD8+ cell ratio shifts of cervical lymph node and spleen cells during pregnancy in rats
Type     članek
Source     J Reprod Immunol
Vol. and No.     Letnik 20, št. 2
Publication year     1991
Volume     str. 165-74
Language     eng
Abstract     Cervical lymph node (CLN) cells and spleen cells were harvested from virgin and pregnant rats bearing syngeneic or allogeneic fetuses at all stages of pregnancy including the pre-implantation period. The specific and non-specific alloreactivity of these cells were analyzed in MLR against mitomycin-C treated paternal strain or unrelated cells. Mitogen stimulation of the cell cultures utilized PHA, Con-A and PWM. Cells bearing T cell markers were labeled in an indirect assay using the monoclonal antibodies W3/25 and MRC OX 8. Specific alloreactivity is enhanced at mid-pregnancy in both cell populations. Non-specific alloreactivity was suppressed in the cervical lymph node cells. Spleen cells demonstrated an increased non-specific alloreactivity and T polyclonal mitogen reactivity (PHA and Con-A) at mid-pregnancy. Reactivity to Con-A was depressed in the early phase and at the end of allogeneic pregnancy in the CLN. The CD4+/CD8+ ratio was very low during all phases of pregnancy.
Descriptors     ANIMALS
ANTIGENS, CD4
ANTIGENS, CD8
CELL DIVISION
CERVIX UTERI
CONCANAVALIN A
IMMUNE TOLERANCE
ISOANTIGENS
LYMPH NODES
LYMPHOCYTE CULTURE TEST, MIXED
PHYTOHEMAGGLUTININS
POKEWEED MITOGENS
PREGNANCY
PREGNANCY, ANIMAL
RATS
RATS, INBRED STRAINS
SPLEEN
T-LYMPHOCYTES
TIME FACTORS