Author/Editor     Arsov, Zoran; Nemec, Marjana; Schara, Milan; Johansson, Henrik; Langel, Uelo; Zorko, Matjaž
Title     Cholesterol prevents interaction of the cell-penetrating peptide transportan with model lipid membranes
Type     članek
Source     J Pept Sci
Vol. and No.     Letnik 14, št. 12
Publication year     2008
Volume     str. 1303-8
Language     eng
Abstract     Interaction of the cell-penetrating peptide (CPP) cysteine-transportan (Cys-TP) with model lipid membranes was examined by spin-label electron paramagnetic resonance (EPR). Membranes were labeled with lipophilic spin probes and the influence of Cys-TP on membrane structure was studied. The influence of Cys-TP on membrane permeability was monitored by the reduction of a liposome-trapped water-soluble spin probe. Cys-TP caused lipid ordering in membranes prepared from pure dimyristoylphosphatidylcholine (DMPC) and in DMPC membranes with moderate cholesterol concentration. In addition, Cys-TP caused a large increase in permeation of DMPC membranes. In contrast, with high cholesterol content, at which model lipid membranes are in the so-called liquid-ordered phase, no effect of Cys-TP was observed, either on the membrane structure or on the membrane permeability. The interaction between Cys-TP and the lipid membrane therefore depends on the lipid phase. This could be of great importance for understanding of the CPP-lipid interaction in laterally heterogeneous membranes, while it implies that the CPP-lipid interaction can be different at different points along the membrane.
Descriptors     CHOLESTEROL
ELECTRON SPIN RESONANCE SPECTROSCOPY
GALANIN
LIPID BILAYERS
MEMBRANES, ARTIFICIAL
RECOMBINANT FUSION PROTEINS
WASP VENOMS