Author/Editor | Arsov, Zoran; Nemec, Marjana; Schara, Milan; Johansson, Henrik; Langel, Uelo; Zorko, Matjaž | |
Title | Cholesterol prevents interaction of the cell-penetrating peptide transportan with model lipid membranes | |
Type | članek | |
Source | J Pept Sci | |
Vol. and No. | Letnik 14, št. 12 | |
Publication year | 2008 | |
Volume | str. 1303-8 | |
Language | eng | |
Abstract | Interaction of the cell-penetrating peptide (CPP) cysteine-transportan (Cys-TP) with model lipid membranes was examined by spin-label electron paramagnetic resonance (EPR). Membranes were labeled with lipophilic spin probes and the influence of Cys-TP on membrane structure was studied. The influence of Cys-TP on membrane permeability was monitored by the reduction of a liposome-trapped water-soluble spin probe. Cys-TP caused lipid ordering in membranes prepared from pure dimyristoylphosphatidylcholine (DMPC) and in DMPC membranes with moderate cholesterol concentration. In addition, Cys-TP caused a large increase in permeation of DMPC membranes. In contrast, with high cholesterol content, at which model lipid membranes are in the so-called liquid-ordered phase, no effect of Cys-TP was observed, either on the membrane structure or on the membrane permeability. The interaction between Cys-TP and the lipid membrane therefore depends on the lipid phase. This could be of great importance for understanding of the CPP-lipid interaction in laterally heterogeneous membranes, while it implies that the CPP-lipid interaction can be different at different points along the membrane. | |
Descriptors | CHOLESTEROL ELECTRON SPIN RESONANCE SPECTROSCOPY GALANIN LIPID BILAYERS MEMBRANES, ARTIFICIAL RECOMBINANT FUSION PROTEINS WASP VENOMS |