Author/Editor     Zorn, B; Ihan, A; Kopitar, AN; Kolbezen, M; Sešek-Briški, A; Meden-Vrtovec, H
Title     Changes in sperm apoptotic markers as related to seminal leukocytes and elastase
Type     članek
Source     Reprod Biomed Online
Vol. and No.     Letnik 21
Publication year     2010
Volume     str. 84-92
Language     eng
Abstract     To elucidate the effects of inflammation on sperm quality, this study analysed classical sperm characteristics, leukocytes and elastase in neat semen, and sperm apoptotic markers, i.e. changes in plasma membrane phospholipid asymmetry, mitochondrial membrane potential (MMP), DNA integrity and intracellular reactive oxygen species (ROS), in semen prepared by density gradient using flow cytometry from 348 men of infertile couples. Increased leukocytes (0.1x10(6)/ml) were associated with a decreased sperm concentration, motility and normal morphology (P0.001). Sperm necrosis and DNA denaturation were increased (31.3 versus 26.6%, P=0.020; 15.5 versus 11.5%, P=0.011, respectively), whereas spermatozoa with normal MMP were decreased (64.1 versus 70.0%, P=0.004). High leukocyte levels (1x10(6)/ml) were not associated with any of the observed sperm parameters. At low elastase concentration (100-290mug/l), DNA denaturation was higher (16.1 versus 10.5%, P=0.024) compared with very low elastase concentration (<100mug/l). A high elastase concentration (290-1000mug/l) was associated with higher ROS index compared with low elastase concentration (1.28 versus 1.01, P=0.016). Slightly increased leukocytes and elastase are associated with slightly poorer sperm characteristics and/or increased sperm necrosis, DNA denaturation and intracellular ROS and decreased MMP.
Descriptors     INFERTILITY, MALE
SEMEN
LEUKOCYTES
APOPTOSIS
CELL MEMBRANE
PHOSPHOLIPIDS
REACTIVE OXYGEN SPECIES
MITOCHONDRIA
FLOW CYTOMETRY
SPERMATOZOA
DNA DAMAGE