Author/Editor | Domanović, Dragoslav; Sever, Matjaž; Vrtovec, Bojan; Ležaić, Luka; Fettich, Jure; Černelč, Peter; Rožman, Primož | |
Title | Preparation of autologous CD34+ cells for intracoronary infusion to patients with end-stage dilated cardiomyopathy | |
Type | članek | |
Source | Bilten za transfuziologiju | |
Vol. and No. | Letnik 55, št. 1-2 | |
Publication year | 2009 | |
Volume | str. 79-83 | |
Language | eng | |
Abstract | Background. We have evaluated the results of the mobilization and collection of autologous peripheral blood stem cells (PBSC) as well as the further selection of CD 34+ cells. The autologous CD34+ cell products were prepared for intracoronary infusion to patients with end-stage dilated cardiomyopathy in order to replace damaged cardiomyocytes and to improve their heart function. Methods and Results. In 36 patients with dilated cardiomyopathy, the PBSCs were mobilized by daily subcutaneous injections of granulocyte colony-stimulating factors (G-CSF) and were collected by small volume leukaphereses as mononuclear cell (MNC) fraction. CD34+ cells were positively selected by the immunomagnetic method. CD34+ cell number target was 90 x 106 per one infusion. The median MNC count before mobilization was 2.65 (2.3 - 3.9) x 109/L. After mobilization it increased approximately 2 times to median value of 6.6 (2.3 - 11.7) x 109/L. The median number of CD34+ cells in patients after mobilization was 125.6 (30.2 - 426.1) x 106/L. Leukapheresis product that contained in median 20.1 (10.7 - 37.9) x109 of MNC and 125.6 (30.2 - 426.1) x106 CD34+ cells. Median collection efficiency of the MNC was 38.3 (9.1 - 74.5) % and 51.3 (23.6 - 81.1) % of CD34+ cells. In the median volume 102 (98 - 115) mL of the selected product the median total number of CD34+ cells in was 95 (49.7 - 154.8) x 106. Conclusions. The PBSC mobilization with G-CSF and the collection by leukapheresis are safe and well tolerated procedures for patients with dilative cardiomyopathy. The immunomagnetic selection of CD34+ cells from the collected PBSCs provides high quality final products for secure intracoronary infusion. Cardiomyopathy patients showed lower responsiveness to PBSC mobilization with G-CSF. | |
Descriptors | HEART FAILURE, CONGESTIVE CARDIOMYOPATHY, CONGESTIVE ANTIGENS, CD34 HEMATOPOIETIC STEM CELL TRANSPLANTATION INFUSIONS, INTRA-ARTERIAL CORONARY VESSELS GRANULOCYTE COLONY-STIMULATING FACTOR LEUKOCYTES, MONONUCLEAR LEUKAPHERESIS PHENOTYPE IMMUNOMAGNETIC SEPARATION BLOOD TRANSFUSION, AUTOLOGOUS |