| Author/Editor | Soukup, T; Smerdu, V; Zacharova, G | |
| Title | Fiber type composition of unoperated rat soleus and extensor digitorum longus muscles after unilateral isotransplantation of a foreign muscle in long-term experiments | |
| Type | članek | |
| Source | Physiol Res | |
| Vol. and No. | Letnik 58, št. 2 | |
| Publication year | 2009 | |
| Volume | str. 253-62 | |
| Language | eng | |
| Abstract | We examined the effects of the unilateral heterochronous isotransplantation on the fiber type composition and myosin heavy chain (MyHC) isoform content of unoperated slow soleus and fast extensor digitorum longus muscles of female inbred Lewis strain rats. Comparison was made between control unoperated muscles of experimental rats (after intramuscular transplantation surgery) with the corresponding muscles of completely naive (unoperated) rats of three age groups (5-, 8- and 14-month-old). This was done in order to ascertain whether these muscles can be used as reliable controls to the transplanted and host muscles for our ongoing grafting experiments. The fiber type composition was determined by assessing the histochemical reaction for myofibrillar adenosine triphosphatase, the MyHC isoform content was determined immunocytochemically using monoclonal antibodies specific to different MyHC isoforms and by sodium dodecyl sulphate polyacrylamide gel electrophoresis. Our experiments show that the heterochronous intramuscular isotransplantation procedure had no significant effect on the fiber type composition and MyHC isoform content of the control unoperated muscles of the experimental rats when compared to the corresponding muscles of the naive animals. Furthermore, the duration and type of isotransplantation did not also lead to differences among corresponding control muscles of experimental animals. We conclude that the unoperated muscles of the experimental rats can be used as controls in our current transplantation project dealing with long-term grafting experiments. | |
| Descriptors | ANIMALS ELECTROPHORESIS, POLYACRYLAMIDE GEL GRAFT SURVIVAL IMMUNOHISTOCHEMISTRY ISOMERISM MUSCLE FIBERS, FAST-TWITCH MUSCLE FIBERS, SLOW-TWITCH MUSCLE, SKELETAL MYOFIBRILS MYOSIN HEAVY CHAINS RATS RATS, INBRED LEW TRANSPLANTATION, HOMOLOGOUS |