Author/Editor		Trontelj, Katja; Ušaj, Marko; Čurin-Šerbec, Vladka; Miklavčič, Damjan
Title		Zlivanje celic z elektrofuzijo
Translated title		Cell electrofusion
Type		članek
Source		Med Razgl
Vol. and No.		Letnik 49, št. 3
Publication year		2010
Volume		str. 247-54
Language		slo
Abstract		This paper describes the phenomena of cell fusion: it' s importance in life processes and the use of electromagnetic field in induced electrofusion. Cell fusion is a common phenom en on in the living organisms during development and later for tissue regeneration. In living organisms cell fusion is specific; regulated by different specialized fusion proteins and protein complexes. The ability of membranes to fuse nonspecifically, induced by an external influence, such as an electric field is, however, important for biotechnology, medicine and research in biology. It enables us to produ ce very useful hybrid cells and their products - monoclonal antibodies, and to study basic mechanisms of cell fusion. Electrofusion is an efficient non viral and non chemical method for cell fusion, where cell membranes are destabilized by means of short, high voltage electric pulses. Optimal values of electrical parameters for fusion have to be determined for each cellline. Besides cell membrane destabilization, contact between cells should be achieved. In research, fusion yield is most of ten determined via fluorescent cell tracker dyes. In hybridoma technology selection media and appropriate mieloma cells are used, so that only hybrid cells survive. Hybridoma yield is, however, much smaller then fusion yield detected by means of fluorescent dyes sin ce only fused cells that survive and proliferate are detected.
Descriptors		CELL FUSION ELECTROPORATION HYBRID CELLS HYBRIDOMAS ANTIBODIES, MONOCLONAL FLUORESCENT DYES