Author/Editor		Stoevesandt, Oda; Vetter, Maike; Kastelic, Damjana; Palmer, Elizabeth A; He, Mingyue; Taussig, Michael J
Title		Cell free expression put on the spot: Advances in repeatable protein arraying from DNA (DAPA)
Type		članek
Source		N Biotechnol
Vol. and No.		Letnik 28, št. 3
Publication year		2011
Volume		str. 282-90
Language		eng
Abstract		We have previously described the 'DNA array to protein array' (DAPA) method for microarraying of proteins expressed by cell-free systems in situ on the array surface. In this technique, a DNA array on one slide acts as the template for generating a protein array on a second slide, mediated by a cell free lysate between the two juxtaposed slides. Here we explore the feature of the repeatability of the technology, in which the same DNA array is reused several times, and use the method to generate a microarray of over 120 diverse proteins. The capabilities of DAPA technology in comparison with other protein array methods are discussed.
Descriptors		DNA PROTEINS CELL-FREE SYSTEM MOLECULAR PROBE TECHNIQUES POLYMERASE CHAIN REACTION