Author/Editor		Kovačič, Borut; Čižek-Sajko, Mojca; Vlaisavljević, Veljko
Title		A prospective, randomized trial on the effect of atmospheric versus reduced oxygen concentration on the outcome of intracytoplasmic sperm injection cycles
Type		članek
Source		Fertil Steril
Vol. and No.		Letnik 94, št. 2
Publication year		2010
Volume		str. 511-9
Language		eng
Abstract		Objective: To assess whether the embryo cultivation at different oxygen tensions had any effect on intracytoplasmic sperm injection (ICSI) outcome. Design: Prospective randomized trial. Setting: University clinical center. Patient(s): Women undergoing ICSI (n 647). Intervention(s): Culturing of embryos, either at 6% CO2, 5% O2, 89% N2, or at 6% CO2 in air. Main Outcome Measure(s): The primary outcome was ongoing pregnancy rates (PR). Secondary outcomes: cumulative PRs, implantation, and embryo quality, for both treatment groups and clinical outcomes for subgroups (optimal cycles, poor responders, older women). Result(s): Although low oxygen resulted in a higher proportion of good day 2 embryos (0.547 +/- 0.021 vs. 0.39 +/- 0.019) and optimal blastocysts (0.162 +/- 0.199 vs. 0.083 +/- 0.128), the ongoing PRs (31.6% vs. 27.1%) and implantation rates (28.8% vs. 25.2%) were similar in both oxygen groups. Low oxygen caused a higher cumulative PR (38% vs. 28.3%) in the main group and a higher PR in the poor responder subgroup (23% vs. 9.8%) with embryo transfers performed mostly on day 3. Conclusion(s): The use of reduced oxygen in IVF is reasonable, irrespective of the duration of embryo culture. It improves embryo development and cumulative PR and is also recommended in poor responding cycles.
Descriptors		FERTILIZATION IN VITRO PREGNANCY RATE OXYGEN