Author/Editor     Jakovac-Strajn, B; Ihan, A; Kopitar, AN; Malovrh, T
Title     Phagocytic activity in blood and proliferation of peripheral blood lymphocytes during the perinatal period in primiparous sows
Type     članek
Source     J Anim Physiol An N
Vol. and No.     Letnik 95, št. 3
Publication year     2011
Volume     str. 328-34
Language     eng
Abstract     The objective of this study was to determine whether phagocytic activity in blood and proliferation of peripheral blood lymphocytes are impaired during perinatal period. The study comprised 18 primiparous sows (Landras x Large White) free from clinical signs of diseases. During the experiment blood samples were collected three times from each sow. Sampling was performed on three different dates, always from all sows at once. At the first date of sampling sows were 21 +/- 3 days before parturition, at the second date +/- 1 day around parturition time and at the third date 21 +/- 3 days after parturition. Phagocytic activity of monocytes and granulocytes was assessed in heparinized whole blood with addition of fluorescein isothiocyanate (FITC)-labelled opsonized bacteria Escherichia coli and the percentage of phagocytes which have ingested bacteria was measured as fluorescence activity by flow cytometry. The percentage of phagocyting monocytes and granulocytes was lowest at parturition (72.6 +/- 16.37, 52.4 +/- 20.59) and significantly increased within the next 21 +/- 3 days (86.5 +/- 6.16, 69.89 +/- 5.80). Similarly, the phytohemagglutinin (PHA) (10 mug/ml) stimulated in vitro lymphocyte response was suppressed by parturition in primiparous sows (p < 0.001).
Descriptors     PHAGOCYTES
PHAGOCYTOSIS
LYMPHOCYTE COUNT
PREGNANCY, ANIMAL
PUERPERIUM
ESCHERICHIA COLI
FLOW CYTOMETRY
SWINE