Author/Editor		Kapoor, Amit; Mehta, Natasha; Esper, Frank; Poljšak-Prijatelj, Mateja; Quan, Phenix-Lan; Qaisar, Natasha; Delwart, Eric; Lipkin, Ian W
Title		Identification and characterization of a new bocavirus species in gorillas
Type		članek
Source		PLoS One
Vol. and No.		Letnik 5, št. 7
Publication year		2010
Volume		str. e11948
Language		eng
Abstract		A novel parvovirus, provisionally named Gorilla Bocavirus species 1 (GBoV1), was identified in four stool samples from Western gorillas (Gorilla gorilla) with acute enteritis. The complete genomic sequence of the new parvovirus revealed three open reading frames (ORFs) with an organization similar to that of known bocaviruses. Phylogenetic analysis using complete capsid and non structural (NS) gene sequence suggested that the new parvovirus is most closely related to human bocaviruses (HBoV). However, the NS ORF is more similar in length to the NS ORF found in canine minute virus and bovine parvovirus than in HBoV. Comparative genetic analysis using GBoV and HBoV genomes enabled characterization of unique splice donor and acceptor sites that appear to be highly conserved among all four HBoV species, and provided evidence for expression of two different NS proteins in all primate bocaviruses. GBoV is the first non-human primate bocavirus identified and provides new insights into the genetic diversity and evolution of this highly prevalent and recently discovered group of parvoviruses.
Descriptors		ANIMALS FECES GENOME, VIRAL GORILLA GORILLA OPEN READING FRAMES PHYLOGENY POLYMERASE CHAIN REACTION