Author/Editor		Gutierrez-Aguirre, Ion; Banjac, Marko; Steyer, Andrej; Poljšak-Prijatelj, Mateja; Peterka, Matjaž; Štrancar, Aleš; Ravnikar, Maja
Title		Concentrating rotaviruses from water samples using monolithic chromatographic supports
Type		članek
Source		J Chromatogr A
Vol. and No.		Letnik 1216, št. 13
Publication year		2009
Volume		str. 2700-4
Language		eng
Abstract		Rotaviruses are the leading cause of diarrhoea in infants around the globe and, under certain conditions they can be present in drinking water sources and systems. Ingestion of 10-100 viral particles is enough to cause disease, emphasizing the need for sensitive diagnostic methods. In this study we have optimized the concentration of rotavirus particles using methacrylate monolithic chromatographic supports. Different surface chemistries and mobile phases were tested. A strong anion exchanger and phosphate buffer (pH 7) resulted in the highest recoveries after elution of the bound virus with 1M NaCl. Using this approach, rotavirus particles spiked in 1l volumes of tap or river water were efficiently concentrated. The developed concentration method in combination with a real time quantitative polymerase chain reaction assay detected rotavirus concentrations as low as 100 rotavirus particles/ml.
Descriptors		CHROMATOGRAPHY, ION EXCHANGE ENVIRONMENTAL MONITORING FECES FRESH WATER METHACRYLATES ROTAVIRUS