Author/Editor		Hafner-Bratkovič, Iva; Bester, Romina; Pristovšek, Primož; Veranič, Peter; Gašperšič, Jernej; Manček-Keber, Mateja; Avbelj, Matevž; Jerala, Roman
Title		Globular domain of the prion protein needs to be unlocked by domain swapping to support prion protein conversion
Type		članek
Source		J Biol Chem
Vol. and No.		Letnik 286, št. 14
Publication year		2011
Volume		str. 12149-56
Language		eng
Abstract		Prion diseases are fatal transmissible neurodegenerative diseases affecting many mammalian species. The normal prion protein (PrP) converts into a pathological aggregated form, PrPSc, which is enriched in the ?-sheet structure. While the high resolution structure of the normal PrP was determined, the structure of the converted form of PrP remains inaccessible to high resolution techniques. In order to map the PrP conversion process we introduced disulfide bridges into different positions within the globular domain of PrP, tethering selected secondary structure elements. The majority of tethered PrP mutants exhibited increased thermodynamic stability, nevertheless they converted efficiently. Only the disulfides which tether subdomain B1-H1-B2 to subdomain H2-H3 prevented PrP conversion in vitro and in prion infected cell cultures. Reduction of disulfides recovered the ability of these mutants to convert, demonstrating that the separation of subdomains is an essential step in conversion. Formation of disulfide-linked proteinase K-resistant dimers in fibrils composed of a pair of single cysteine mutants supports the model based on domain-swapped dimers as the building blocks of prion fibrils. In contrast to previously proposed structural models of PrPSc suggesting conversion of large secondary structure segments, we provide evidence for the conservation of secondary structure elements of the globular domain upon PrP conversion. Previous studies already showed that dimerization is the rate-limiting step in PrP conversion. We show that separation and swapping of subdomains of the globular domain is necessary for conversion. Therefore, we propose that domain-swapped dimer of PrP precedes amyloid formation and represents a potential target for therapeutic intervention.
Descriptors		PRION DISEASES PRIONS PRPC PROTEINS PRPSC PROTEINS DIMERIZATION TRANSFECTION PLASMIDS ESCHERICHIA COLI TRANSDUCTION, GENETIC MODELS, STRUCTURAL MICROSCOPY, ELECTRON CIRCULAR DICHROISM CYSTEINE MUTATION FLOW CYTOMETRY MICROSCOPY, ATOMIC FORCE